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TC-4000 and TC-5000 Thermal Cyclers

The TC-4000 and TC-5000 offer high performance and high sample throughput for maximum flexibility and economy, and are ideal for a larger number of parallel samples. Both units offer:

• Versatile block format: user-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60x0.5ml, 96x0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ)
• Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
• Temperature range of 4°C to 99°C
• 8 Peltiers: each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
• Incremental/decremental time and temperature for applications such as Touchdown PCR
• The TC-4000 and TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software

The TC-4000 is one of the most affordable full sized thermal cyclers in the market, flexible for all your protocols and easy on your budget.

•Intuitive programming: 4-line display with intuitive, simple-to-use software with alphanumeric programming, password protection, simple copy/edit functions and much more.
•Excellent heating rate of 2.6°C/sec and block uniformity of ±0.3°C at 50°C, ensuring optimal reproducibility

The TC-5000 gradient thermal cycler enables you to optimize your experiments at the `touch of a screen', with the same mechanical features as the TC-4000.

• `Real-time' graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualization of your experiment's status.
•User-friendly programming interface provided by a 115 x 90mm touch-sensitive screen.
•Excellent heating rate: A maximum heating rate of 3°C/sec and block uniformity of ±0.3°C at 50°C with or without a compromise!
•The most linear gradient cycler on the market, with an amazing range of 30°C.
•The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.
•Gradient range 20°C to 70°C, maximum 30°C
•Decremental/incremental time and temperature

Technical Specifications

Parameters TC-5000 TC-4000
0.5ml block capacity 60 60
0.2ml block capacity 96 96
384-well block Yes Yes
In-situ block Yes Yes
Temperature range 4°C to 99°C 4°C to 99°C
Gradient range 20°C to 70°C NA
Maximum gradient¹ 30°C (16°C for 384 block) NA
Maximum ramp rate² 3.0°C/sec 2.6°C/sec
Block uniformity at 50°C <±0.3°C <±0.3°C
Selectable heated lid temperature 100°C - 115°C 100°C - 115°C
Heated lid enable/disable Yes Yes
Over-temperature cut-out Yes Yes
Number of programs³ 50 80
Programming Touchscreen 4-line alphanumeric
Graphical display Real-Time Graph NA
Maximum hold time 99 hrs 18 hrs
Minimum hold time 1 sec 1 sec
Auto restart on power failure Yes Yes
Dimensions L x W x H 420 x 220 x 260mm (16.5 x 8.7 x 10.2") 420 x 220 x 260mm (16.5 x 8.7 x 10.2")
Voltage Dual voltage 230/115V 50-60Hz Dual voltage 230/115V 50-60Hz
Power 620W 620W
Shipping weight 14kg (30.8 lbs) 13kg (28.6 lbs)

Ordering Information

Product Code Description
FTC4/05 TC-4000 thermal cycler for 60 x 0.5ml microtubes, 120V / 230V
FTC4/H02 TC-4000 thermal cycler for 96 x 0.2ml microtubes or 96-wellplates, 120V / 230V
FTC4/F02 TC-4000 thermal cycler for 96 x 0.2ml microtubes or 96-well fully skirted plate, 120V / 230V
FTC4/FLAT TC-4000 thermal cycler for In-situ, 120V / 230V
FTC4/384 TC-4000 thermal cycler for 384-well microplate, 120V / 230V
FTC5/05 TC-5000 Gradient thermal cycler for 60 x 0.5ml microtubes, 120V / 230V
FTC5/H02 TC-5000 Gradient thermal cycler for 96 x 0.2ml microtubes or 96-well plate, 120V / 230V
FTC5/F02 TC-5000 Gradient thermal cycler for 96 x 0.2ml microtubes or 96-well fully skirted plate, 120V / 230V
FTC5/FLAT TC-5000 Gradient thermal cycler for In-situ (non-gradient), 120V / 230V
FTC5/384 TC-5000 Gradient thermal cycler for 384-well microplate,120V / 230V

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

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